山羊GDF9基因在卵丘卵母细胞复合体体外成熟过程中的表达

Expression of Growth Differentiation Factor 9 in Goat COCs during in vitro Maturation

本研究采用实时荧光定量PCR技术,从mRNA水平探讨了山羊卵丘卵母细胞复合体体外成熟(IVM)过程中(0、6、12、18、24和27h)GDF9基因的相对表达变化,分析其与卵丘细胞扩散之间的关系。结果表明,GDF9 mRNA在山羊卵丘卵母细胞复合体体外成熟过程中均有表达,且在成熟培养初期表达量较低,其表达峰值出现在IVM12h,与卵丘细胞开始扩散的时间一致,之后又有所下降。由此推测,GDF9基因在山羊卵丘扩散和卵母细胞体外成熟过程中起着重要的作用。

To analyze the relationship between GDF9 gene and expansion of cumulus-oocyte complex （COCs）, the expression of GDF9 in goat COCs during oocytes in vitro maturation （IVM） （0, 6, 12, 18, 24 and 27 h） was detected by fluorescent quantitative RT-PCR method. The result showed that GDF9 mRNA were expressed at low levels in immature oocytes and increased to the highest level at 12 h of IVM, which coincides with the time of cumulus cell expansion, then declined slowly during IVM, with a slight increase at the end of IVM. The expression pattern of GDF9 suggested that it might be important in cumulus expansion and oocyte maturation of goat.