猪Hepcidin基因的克隆与序列分析

Cloning and Sequence Analysis of Gene Hepcidin from Pig

通过RT-PCR技术从猪肝脏总RNA中扩增出Hepcidin基因的编码序列,将该序列及其推导的氨基酸序列与其它17个已知物种的相应序列进行同源性分析,并构建系统进化树。结果表明,克隆得到的Hepcidin基因cDNA序列长278 bp,编码82个氨基酸,预测的蛋白分子质量和等电点分别为8.76 ku和9.06;与已知牛的Hepcidin序列同源性最高,达81.71%,不同物种Hepcidin蛋白具有高度的保守性,Hepcidin蛋白物种进化树符合物种进化规律。本研究为今后阐明Hepeidin基因表达特性、表达产物理化性质、抗菌活性及其相关功能等奠定基础。

In this study,Hepcidin encoding region of Sus scrofa liver was studied by RT-PCR method,and the homology of Hepcidin encoding region and Hepcidin amino acid sequence was analyzed among Sus scrofa and 17 other species.The phylogenetic tree of Hepcidin amino acid sequence was established.The results showed that the length of the cloned cDNA was 278 bp,encoding 82 amino acids,and the forecasted protein molecular weight and isoelectric points were 8.76 ku and 9.06 respectively.The homology of Hepcidin encoding region was 81.71% between Sus scrofa and Bos Taurus.The phylogenetic tree conformed to the law of species evolution.