期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

靶向趋化因子受体5短肽的筛选及活性初步分析

Screening short peptides affinity to chemokine receptors CCR5 and analyzing their activity
下载PDF
导出
摘要 目的筛选人趋化因子受体5(CCR5)的亲和短肽。方法采用噬菌体十二肽库对稳定表达CCR5的中国仓鼠卵巢(CHO)细胞(CHO/CCR5)进行筛选,得到30个阳性克隆,通过酶联免疫吸附试验(ELISA)分析,发现其中17个克隆含有AFDWTFVPSLIL基序。结果含有该序列的噬菌体和合成肽AFDWTFVPSLIL能阻止单克隆抗体2D7与CHO/CCR5细胞的结合;并能竞争性地抑制RANTES对CHO/CCR5细胞的结合。结论小分子肽AFDWTFVPSLIL能特异性地结合CCR5,并有可能成为CCR5的拮抗剂。 Objective To screen the short peptides affinity to chemokine receptors CCR5（CCR5）. Methods The peptides that bind to Chinese hamster ovarian（CHO） cells that stably expressed CCR5（CHO/CCR5） were identified from Ph.D.-12 phage display peptide library.30 positive clones were obtained.The ELISA technique was used to analyze the gene sequence.17 of the 30 positive clones contained with AFDWTFVPSLIL gene sequence. Results The phage that contained this gene sequence and synthetic peptide AFDWTFVPSLIL blocked the binding of monoclonal antibody 2D7 to CHO/CCR5 cells,and could competitively inhibit the binding of RANTES to CHO/CCR5 cells. Conclusion Small peptide AFDWTFVPSLIL can specifically bind to CCR5 and might be the antagonist of CCR5.
作者 谢惠芳 谭志军 邓霞林
机构地区 湖南省祁阳县人民医院检验科
出处 《右江民族医学院学报》 2010年第3期299-302,共4页 Journal of Youjiang Medical University for Nationalities
关键词 肽类 受体 CCR5 peptides receptors CCR5
分类号 Q782 [生物学—分子生物学] Q516 [生物学—生物化学]
  • 相关文献

参考文献18

  • 1Berger EA, Murphy PM, Farber JM. Chemokine receptors as HIV- 1 coreceptors: roles in viral entry tropism and disease [J]. Annu Rev Immunol, 1999,17:657 - 700.
  • 2Belnoue E, Kayibanda M, Deschemin JC, et al. CCR5 deficiency decreases susceptibility to experimental cerebral malaria [J]. Blood, 2003,101 (11 ) : 4253 - 4259.
  • 3Trebst C, Sorensen TL, Kivisakk P, et al. CCR1 +/CCR5 + mononuclear phagocytes accumulate in the central nervous system of patients with multiple sclerosis [ J ]. Am J Pathol,2001,159(5) : 1701 - 1710.
  • 4Mack M, Bruuhl H, Gruber R, et al. Predominance of mononuclear cells expressing the chemokine receptor CCR5 in synovial effusions of patients with different forms of arthritis [J]. Arthritis Rheum,1999,42(5) :981-988.
  • 5Shields PL, Morland CM, Salmon M, et al. Chemokine and chemokine receptor interactions provide a mechanism for selective T cell recruitment to specific liver compartments within hepatitis C- infected liver [J]. J Immunol, 1999,163(11) :6236 - 6243.
  • 6Hancock WW, Gao W, Faia KL, et al. Chemokines and their receptors in allograft rejection [J]. Curr Opin Immunol,2000,12(5) :511 - 516.
  • 7Strizki JM, Xu S, Wagner NE, et al. SCH - C (SCH 351125), an orally bioavailable, small molecule antagonist of the ehemokine receptor CCR5, is a potent inhibitor of HIV- 1 infection in vitro and in vivo [J]. Proc Natl Acad Sci USA,2001,98(22) :12718 - 12723.
  • 8Yanofsky SD, Baldwin DN, Butler JH, et al. High affinity type Ⅰ interleukin 1 receptor antagonists discovered by screening recombinant peptide libraries [J]. Proc Natl Acad Sci USA, 1996,93 (14) : 7381 - 7386.
  • 9Wrighton NC, Farrell FX, Chang R, et al. Small peptides as potent mimetics of the protein hormone erythropoietin [J]. Science, 1996,273(5274) :458 - 464.
  • 10Nicklin SA, White SJ, Watkins SJ, et al. Selective targeting of gene transfer to vascular endothelial cells by use of peptides isolated by phage display [J]. Circulation,2000, 102(2) :231 - 237.
右江民族医学院学报
2010年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部