甲胎蛋白及增殖细胞核抗原在自然流产中的表达及意义

The study on the expression and significance of AFP and PCNA in spontaneous abortion

目的检测自然流产患者和正常妊娠妇女羊水甲胎蛋白(alpha-fetoprotein,AFP)的含量及胎盘的增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)的表达,探讨AFP和PCNA与自然流产发生的关系。方法选取2008年10月至2009年12月潍坊市妇幼保健院计划生育科64例自然流产患者为研究组,其中先兆流产32例,稽留流产32例。32例正常妊娠妇女为对照组。用电化学发光法检测各组羊水AFP含量。免疫组织化学SP法检测胎盘组织PCNA的表达。结果正常妊娠组、先兆流产组及稽留流产组羊水AFP分别为(8356±607)μg/L,(7024±498)μg/L,(5638±533)μg/L,各组之间比较差异有显著性(P<0.05)。正常妊娠组、先兆流产组及稽留流产组PCNA分别为(56.49±2.17)%,(48.96±1.43)%,(39.53±1.74)%,各组之间比较差异有显著性(P<0.05)。3组中胎盘PCNA增殖指数与同组羊水AFP显著相关(P<0.05)。结论自然流产的发生可能与AFP和PCNA的表达水平异常降低有关。

Objective To study the relationship between alpha-fetoprotein （AFP）,proliferation cell nuclear antigen （PCNA） in amniotic fluid and spontaneous abortion through detecting the level of AFP and PCNA in spontaneous abortion patients and normal pregnancy women. Methods sixty-four spontaneous abortion patients were collected as study group,who was further divided into 2 subgroups corresponding to threatened abortion with 32 patients and missed abortion with 32 patients. The other 32 cases of normal pregnancy were as control group. The level of AFP in amniotic fluid was determined with chemiluminescence immunoassay. The expression of PCNA in placenta tissue was examined SP immune-histochemistry technique. Results The level of amniotic fluid AFP was（8 356±607）μg/L in normal pregnancy group,（7 024±498） μg/L in threatened abortion group,（5 638±533）μg/L in missed abortion group respectively. There were significant differences among three groups （P〈0.05）. The positive rate of PCNA expression was（56.49±2.17）% in normal pregnancy group,（48.96±1.43）% in threatened abortion group,（39.53±1.74）% in missed abortion group recpectively. There were significant differences among three groups （P〈0.05）. Proliferation index（PI） of PCNA was closely related to level of amniotic fluid AFP in every group （P〈0.05）. Conclusion The results showed that low expression of AFP and PCNA may have relationship with spontaneous abortion.