HPLC-ELSD法测定益气维血颗粒中黄芪甲苷的含量

HPLC-ELSD method for the determination of blood Qi-dimensional particle content of Astragaloside

目的:建立益气维血颗粒中黄芪甲苷的含量测定方法。方法:采用HPLC-ELSD法,CAPCELLPAKC18柱(250mm×4.6mm,5μm),以乙腈-水(36∶64)为流动相,流速为0.8mL/min,氮气流量为3.2L/min,漂移管温度为105℃,柱温为30℃。结果:黄芪甲苷在0.2555-2.044μg范围内进样量与峰面积呈良好的线性关系,r=0.9997,平均回收率为102.6%,RSD%=1.2%(n=6)。结论:本方法准确、重现性好,可用于益气维血颗粒的质量控制。

Objective：Qi-dimensional particles in the blood of astragaloside Determination.Methods：HPLC-ELSD method,CAPCELL PAK C18 column（250 mm×4.6 mm,5 μm）,acetonitrile-water（36：64） as mobile phase,the flow rate is 0.8 mL/min,nitrogen flow rate for 3.2 L/min,drift tube temperature is 105℃,the column temperature is 30℃.Results：astragaloside 0.2555-2.044 μg sample volume within the peak area and showed a good linear relationship,r=0.9997,average recovery is 102.6%,RSD%=1.2%（n=6）.Conclusion：This method is accurate,reproducible and can be used for Qi-dimensional quality control of blood particles.