胡椒碱对海人藻酸致惊作用的影响

Effects of piperine on convulsive seizures induced by kainic acid

[目的]观察胡椒碱的致惊作用并分析其机制。[方法]小鼠和家兔的实验性癫痫模型;利用氨基酸自动分析仪检测小鼠脑内氨基酸含量;大鼠脑片谷氨酸释放实验。[结果]胡椒碱对小鼠脑室注射(icv)海人藻酸(KA)引起的阵挛性惊厥有较强的对抗作用,其半数有效量(ED50)为24.0(18.7～33.7)mg/kg。对家兔海马注射KA引起的异常脑电放电有一定的抑制作用。明显降低小鼠脑内谷氨酸(Glu)和天冬氨酸(Asp)含量,并且能够降低Glu+Asp/γ-氨基丁酸(GABA)的比值。10-6 mol的胡椒碱对大鼠大脑皮质脑片预载的3H-Glu的释放有明显的抑制作用。[结论]胡椒碱有较强的抗实验性癫痫作用,其机制可能与降低了中枢神经系统兴奋性氨基酸(EAA)的功能有关。

[Objective] To study the effects of piperine on several experimental epilepsy models and analyze it＇s mechanisms.[Methods] Experimental epilepsy in mice and rabbits was induced.The concentration of amino acid was determined by automatic analyzer in brain of mice.The releasing experiment of glutamic acid in cortex slice of rats was performed.[Results] Piperine had obvious antagonism against clonospasm induced by KA（icv） in mice,the ED50 was 24.0 mg/kg.It could also prolong the latency of abnormal ECoG and decrease the continued time of abnormal ECoG induced by intrahippocampal injection of KA in rabbits.Piperine could reduce the levels of Glu and Asp in mice brain,so it could also decrease the Glu＋Asp/GABA ratio.It could not alter the levels of GABA.Piperine（10-6 mol） It could obviously decrease the release of proloaded [3H]-Glu evoked by high（k＋） in cortex slice in rat.[Conclusion] Piperine has a potent antagonistic effect on experimental epilepsy in mice and rabbits.The mechanism of the anticonvulsant action of piperine is related to the function of EAA in central nerve system.