改进型实验性左侧精索静脉曲张的建立及其对大鼠卵泡刺激素及抑制素B的影响

Improved experimental varicocele increases follicle-stimulating hormone and reduces inhibin B in rats

目的:探讨改进型实验性左侧精索静脉曲张(ELV)对大鼠血清卵泡刺激素(FSH)及抑制素B(InhB)的影响。方法:利用改进的方法建立青春期SD大鼠左精索静脉曲张的模型30只;假手术组SD大鼠30只作对照组。术后3个月,取大鼠血清,ELISA法分别检测FSH、InhB的浓度。结果:实验组大鼠血清中FSH浓度[(37.56±9.72)ng/ml]与对照组[(26.69±5.33)ng/ml]相比升高,具有统计学意义(P<0.05);而实验组大鼠血清中InhB的浓度[(349.93±99.48)pg/ml]与对照组[(768.83±146.96)pg/ml]相比下降,具有统计学意义(P<0.05)。结论:改进型ELV致FSH升高及InhB下降,这些变化可能是影响生育能力的机制之一。

Objective： To evaluate the effects of improved experimental left varicocele （ELV） on follicle-stimulating hormone （FSH） and inhibin B （InhB）. Methods： ELV models were established by the improved method in 30 SD rats, and another 30 were included in a sham operation group as controls. Three months after the operation, the concentrations of the FSH and InhB were assayed by ELISA. Results ： The concentration of FSH in the serum was significantly higher in the experimental group （ [ 37.56 ±9.72 ] ng/ml） than in the control （ [26.69 ± 5.331 ng/ml） （P 〈0.05）, while that of InhB significantly lower in the former （ [349.93 ±99.48 ] pg/ml） than in the latter （ [ 768.83± 146.96 ] pg/ml） （ P 〈 0.05 ）. Conclusion ： Improved ELV can increase FSH and reduce InhB in rats, which may be associated with subfertility.