Rac1在垂体腺瘤组织中的表达及意义

Expression and significance of Rac1 in pituitary tumors

目的探讨Rac1蛋白及mRNA在垂体腺瘤组织中的表达情况。方法应用免疫组化技术、RT-PCR方法检测82例垂体腺瘤标本中Rac1蛋白及mRNA的表达情况,包括29例侵袭性垂体腺瘤和53例非侵袭性垂体腺瘤。结果免疫组化结果显示:垂体腺瘤Rac1标记指数(LI)为3.34%～53.7%,其中侵袭性垂体腺瘤LI平均为33.5%,非侵袭垂体腺瘤LI平均为8.2%,两者差异具有统计学意义(P<0.01)。RT-PCR显示:Rac1mRNA灰度比侵袭性垂体腺瘤为0.891±0.172,非侵袭性垂体腺瘤为0.372±0.181,两者差异具有统计学意义(P<0.01)。结论 Rac1与垂体腺瘤的侵袭能力密切相关,Rac1过表达可增强垂体腺瘤的侵袭能力并导致临床预后不良。

Objective To evaluate the expression of Rac1 protein and mRNA in pituitary tumors.Methods The expression of Rac1 protein and mRNA in 82 patients with pituitary tumors,including 29 invasive pituitary tumors and 53 non-invasive pituitary tumors were studied by immunohistochemistry technique and RT-PCR.Results Rac1 labeling index （LI） of pituitary tumors confirmed by immunohistochemistry was 3.34%-53.7%.The average Rac1 LI in invasive and non-invasive pituitary tumors was 33.5% and 8.2% respectively.The difference in Rac1 LI between invasive and non-invasive pituitary tumors was statistically significant （P0.01）.Gray-scale ratio of Rac1 mRNA was showed by RT-PCR：0.891 ± 0.172 in invasive pituitary tumors and 0.372 ± 0.181 in non-invasive pituitary tumors.The difference in expression of Rac1 mRNA between the two groups was statistically significant （P0.01）.Conclusions A close relationship exists between Rac1 and invasion ability of pituitary tumors.Overexpression of Rac1 may enhance invasion ability and result in a poor prognosis.