5-氨基乙酰丙酸光动力疗法诱导人宫颈癌细胞株Hela和Siha凋亡的研究

Effects of photodynamic therapy with 5-aminolevulinic acid on apoptosis of human cervical carcinoma Hela and Siha cells in vitro

目的探讨5-氨基乙酰丙酸结合光动力疗法对人宫颈腺癌Hela细胞株和鳞癌Siha细胞株凋亡的影响及其相关机制。方法噻唑蓝(MMT)法检测光动力疗法对人宫颈癌细胞株Hela和Siha增殖的影响,并比较不同的抑制作用。Annexin V-FITC/PI双染法检测光动力疗法对Hela和Siha细胞株凋亡的影响。免疫细胞化学法检测光动力疗法对两种细胞Her-2/neu基因的蛋白表达影响。结果 Hela比Siha细胞株对光动力治疗更敏感。5-氨基乙酰丙酸2mmol/L、10J/cm2激光剂量孵育为光动力疗法体外杀伤Hela和Siha细胞株较理想的实验条件,在该条件下Hela、Siha细胞的IC50分别为0.724、1.206mmol/L。光动力疗法可显著诱导Hela和Siha细胞凋亡,并抑制Hela和Siha细胞Her-2/neu基因的蛋白表达。结论光动力治疗在体外对人宫颈腺癌Hela细胞株和鳞癌Siha细胞株有明显的增殖抑制作用,还能诱导两种细胞发生凋亡,其机制可能与抑制Her-2/neu基因表达有关。

Objective To study effects and mechanisms of 5-ALA-PDT（5-aminolevulinic acid-photodynamic therapy） on apoptosis of cervical adenocarcinoma Hela and squamous cell carcinoma Siha cells in vitro. Methods MTT assay and AnnexinⅤ-FITC/PI double staining assay were applied to investigate the effects of 5-ALA-PDT on proliferation and apoptosis of Hela and Siha cells respectively. Effects of 5-ALA-PDT on expression of Her-2/neu in the two cell lines were detected by immunocytochemistry. Results Hela cells were more sensitive to 5-ALA-PDT than Siha cells. 2mmol/L 5-ALA and a light dose of 10J/cm2 were the optimal condition for killing of the Hela and Siha cell lines in vitro by 5-ALA-PDT,under which,the 50% inhibition concentrations （IC50） of 5-ALA-PDT for the Hela and Siha cell lines were 0.724mmol/L and 1.206mmol/L respectively. 5-ALA-PDT remarkably induced apoptosis of Hela and Siha cells and inhibited expression of Her-2/neu protein in the two cell lines. Conclusion 5-ALA-PDT has patent proliferation-inhibiting and apoptosis-promoting effects on the cervical carcinoma Hela and Siha cells in vitro. The mechanism may be related to the down-regulation of Her-2/neu expression.