基质金属蛋白酶-2和趋化因子受体4在肾透明细胞癌组织中的表达及其临床意义

Expressions of MMP-2/CXCR4 and their clinical significance in renal clear cell carcinoma tissues

目的探讨基质金属蛋白酶-2(MMP-2)和趋化因子受体4(CXCR4)在肾透明细胞癌组织中的表达及其与临床病理因素的关系。方法应用免疫组化PV6000法检测79例肾透明细胞癌(RCCC)组织和43例正常肾组织中MMP-2和CXCR4的表达。结果 79例RCCC中MMP-2和CXCR4的阳性表达率为73.4%、77.2%,高于在正常肾组织中的阳性表达率(16.3%,14.0%),差异有统计学意义(χ2=36.520、45.009,P<0.01),其表达与RCCC的临床分期和淋巴结转移有关(χ2=3.978～5.657,P<0.05)。MMP-2和CXCR4的表达呈正相关(r=0.561,P<0.01)。结论 MMP-2和CXCR4在肾透明细胞癌组织中呈高表达,其与肿瘤的发生、发展、转移及临床病理参数之间有相关性,可能在肿瘤的进展过程中扮演重要角色。

Objective To investigate expressions of matrix metalloproteinase-2（MMP-2） and CXC chemokine receptor 4（CXCR4） in renal clear cell carcinoma（RCCC） tissues and their relationship with clinicopathological characteristics. Methods Expressions of MMP-2 and CXCR4 in 79 specimens of renal clear cell carcinoma tissues and 43 specimens of normal renal tissues were detected by immunohistochemical PV-6000 technique. Results The positive rates of MMP-2 and CXCR4 in renal clear cell carcinoma tissues were significantly different from those in normal renal tissues（χ2=36.520,45.009,P0.01）. Furthermore,expressions of MMP-2 and CXCR4 in renal clear cell carcinoma tissues had a significant relationship with clinical stages of tumor and lymph node metastasis（χ2=3.978-5.657,P0.05） while not with gender and age of patients and differentiation of cancer （χ2=0.000-2.321,P0.05）. Positive expression of MMP-2 was positively related to that of CXCR4 in renal clear cell carcinoma （r=0.561,P0.01）. Conclusions Expressions of MMP-2 and CXCR4 in renal clear cell carcinoma tissues are significantly related to the genesis,development,metastasis and clinicopathologic parameters of tumors,and may play an important role in the progression of renal clear cell carcinoma.