曲格列酮对胰岛β细胞胰岛素分泌的影响及机制研究

Effect of troglitazone on insulin secretion in pancreatic β-cells and its mechanism

研究曲格列酮对胰岛β细胞（MIN6细胞株）胰岛素分泌的影响,并探讨其机制.10μmol/L曲格列酮短期抑制大鼠胰岛和MIN6细胞的葡萄糖刺激的胰岛素分泌（GSIS,P〈0.01）,增加AMP活化的蛋白激酶（AMPK）、乙酰辅酶A羧化酶（ACC）的磷酸化水平（均P〈0.01）,而AMPK抑制剂复合物C可使其AMPK、ACC的磷酸化水平以及胰岛素分泌完全恢复.

The effect of troglitazone on glucose-stimulated insulin secretion （GSIS） in pancreatic β-cells and its mechanism were investigated.10 μmol/L troglitazone had no effect on basal insulin secretion,but significantly decreased GSIS and stimulated AMP-activated protein kinase （AMPK） and acetyl-CoA carboxylase （ACC） phosphorylations （all P〈0.01）.These reactions were completely reversed by AMPK inhibitor compound C,suggesting that the troglitazone acutely inhibits insulin secretion via stimulating AMPK activity in beta cells.