肾素基因G10631A和血管紧张素原基因C521T多态性与脑梗死的关系

Association between gene polymorphisms of rennin gene G10631A and angiotensinogen gene C521T and cerebral infarction

目的研究肾素(REN)基因G10631A、血管紧张素原(AGT)基因C521T多态性与脑梗死的关系。方法采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法检测82例脑梗死患者和89名正常对照者的REN基因10631位点、AGT基因521位点的多态性,并用Logistic回归分析两基因多态性与脑梗死的关系。观察两组基因单倍型的分布。结果脑梗死组REN基因10631 AA基因型(31.7%)、A等位基因(49.4%),AGT基因521TT基因型(22.0%)、T等位基因频率(28.0%)显著高于正常对照组(10.1%、30.3%、6.7%、11.8%)(均P<0.05)。Logistic回归分析显示,REN基因10631AA基因型、AGT基因521TT基因型增加脑梗死的发生概率,发病的相对危险度(OR)分别为2.798、3.492(均P<0.05)。脑梗死组基因单倍型521T-10631A的分布频率明显高于正常对照组(P<0.005)。结论 REN基因10631AA基因型和A等位基因、AGT基因521TT基因型和T等位基因可能为脑梗死的易患因素;单倍型521T-10631A可能是脑梗死发病的遗传危险因素。

Objective To study the relationship between the gene polymorphisma of rennin（REN） gene G10631A and angiotensinogen（AGT） gene C521T and cerebral infarction.Methods Polymerase chain reaction（PCR）-restriction fragment length polymorphism（RFLP） was used to detected the gene polymorphisms of REN gene 10631 site,AGT gene 521 site in 82 cerebral infarction patients and 89 normal controls.The relationship between polymorphisms of the two genes and cerebral infarction were analyzed by Logistic regression analysis.The distribution of gene haplotype in the two groups was observed.Results REN gene 10631 AA genotypic（31.7%） and A allele（49.4%）,AGT gene 521 TT genotypic（22.0%） and T allele（28.0%） in cerebral infarction group were significantly higher than those in normal control group（10.1%,30.3%,6.7%,11.8%）（all P〈0.05）.Logistic regression analysis showed that REN gene 10631 AA genetype and AGT gene 521 TT genetype increased the incidence of cerebral infarction,and the pathogenetic relative risk rates（OR） were respectively 2.798 and 3.492（all P〈0.05）.The distribution rate of haplotype 521T-10631A in cerebral infarction group was significantly higher than normal control group（P〈0.005）.Conclusions REN gene 10631AA genetype and A allele,AGT gene 521 TT genetype and T allele may be susceptible factors;haplotype 521T-10631A may be the pathogenetic heredity risk factor of cerebral infarction.