脑膜癌病的脑脊液细胞学特征

Cytologic characteristic of cerebrospinal fluid in meningeal carcinomatosis

目的探讨脑膜癌病的脑脊液细胞学特征。方法对4例脑膜癌病患者行腰椎穿刺脑脊液细胞学检查,其中2例行免疫细胞化学检查。脑脊液细胞学检查采用粟氏玻片离心法,MGG染色,免疫细胞化学检查采用酶标聚合物法(LDP法)。结果 4例患者经脑脊液细胞学检查均发现肿瘤细胞,细胞成团聚集或散在分布,大小不一,形态各异,胞膜有突起,胞浆深染,核浆比例增大,核仁明显。免疫细胞化学检查示广谱角蛋白(CKpan)阳性1例。结论脑膜癌病的脑脊液细胞学主要特征为有异常形态的肿瘤细胞,这是脑膜癌病诊断的主要依据,结合免疫细胞化学检查有助于明确肿瘤细胞的性质。

Objective To investigate the cytologic characteristic of cerebrospinal fluid（CSF） in meningeal carcinomatosis（MC）.Methods Through lumbar puncture,the CSF was tested by routine cytologic examination in 4 patients with MC,and by immunocytochemical examination in 2 cases of them.Su＇s centrifugal and MGG staining methods were used in the cytologic examination of CSF,and LDP staining method was used in the immunocytochemistry.Results Tumor cells were found in all of the 4 cases by cytologic examination of CSF.Tumor cells diffused or gathered with different types and irregularation size.They had prominence of cytomembrane,trachychromatic cytoplasm,high ratio of nuclear to cytoplasmic and prominent nucleoli.The immunocytochemistry examination indicated that CKpan positive was found in 1 case.Conclusions The cytologic characteristic of CSF in MC is the morphological abnormality tumor cell,which is the main basis to diagnose MC.And then it will be helpful to clarify the quality of tumor cells by combining immunocytochemistry.