摘要
目的:探讨自组装纤维支架肽RADA16在皮肤烫伤中的治疗作用。方法:在SD大鼠背部以电力机械方法制造深Ⅱ度烧伤模型,对照治疗组以0.9%NaCl溶液代替,烧伤后按时换药,并在不同时间段(伤后7、10、14d分别取烧伤修复创面的皮肤组织进行免疫组化染色,记录创面碱性成纤维细胞生长因子(bFGF)及表皮细胞生长因子(EGF)的表达,并以图像处理系统软件记录生长因子表达的半定量检测。比较了短肽处理组及空白对照组创面生长因子表达的灰度值。结果:免疫组织化学结果显示,在烧伤修复的不同修复期,短肽处理组bFGF和EGF均明显表达在新生表皮组织中,与空白对照组比较,差异均有统计学意义(P<0.05)。结论:自组装纤维支架肽对烧伤皮肤生长因子的表达具有促进作用。
Objective: To investigate therapeutic effects of self-assembling peptide RADA16-I nanofiber scaffold. Methods: Performed a deep demal partial thickness burn model at the dorsal skin of female Sprague-dawley rats with an electrical scald machine. On the 7th, 10th, 14th day after injury, the wound specimens were removed for immunohistochemical evaluation. Digital photomicrographs of the FGF and EGF-positive tissue were taken and transformed into image analysis software to estimate the intensity of EGF and FGF protein expression with integral optical density. Results: Immunohistochemistry showed the bFGF and EGF were obviously expressed in nascent tissue such as epidermis and glands when wounds were treated with the RADA16-I after injury, compared the blank control group ,the difference was statistically significant (P0.05). Conclusion: There is promoting effect of self-assembling peptide to burn skin growth factor expression.
出处
《中国当代医药》
2010年第18期22-23,共2页
China Modern Medicine