采用ARMS分析口腔脱落细胞ApoE基因型

Analysis of ApoE Genotypes of Exfoliative Cells from Oral Cavity by the Amplification Refractory Mutation System

以口腔脱落细胞ＤＮＡ为模板，采用扩增不应突变系统（ＡＲＭＳ），设计了等位基因４个特异性寡核苷酸引物和一个公共引物，分析了７４例个体ＡｐｏＥ３个常见共显性等位基因∈２、∈３和∈４。这一系统扩增出１８１ｂｐ和３１９ｂｐ两种ＡｐｏＥ基因顺序，当携某一等位基因的模板ＤＮＡ与相应的等位基因特异性寡核着酸引物及公共引物反应时，扩增出相应的基因顺序。省略了限制性核酸内切酶的消化或等位基因特异性寡核苷酸探针的杂交。方法简单、可靠、非放射性。

We analyzed apolipoprotein E (ApoE ) genotypes of exfoliative cells from oral cavity in 74 subjects by the am-plification refractory mutation system (ARMS ). By using four allele-specific oligonucleotide primers and one iscommon primer, the other can identify the three alleies of the ApoE genetic polymorphism , and respec-tirely. The system amplifies two sequences of the ApoE gene, one of 181bp and the other 319bp. These sequences are amplified when DNA containing a particular allele is incubated with its allele-specific oligonucleotide primer anda common primer. The method is simple, reliable, and nonisotopic and obviate the need for digestion with restric-tion endonucleases or for hybridization with allele-specific oligonucleotide probes.