酸性成纤维细胞生长因子脂质体的制备及体外释药动力学

Preparation of aFGF liposomes and their release behavior in vitro

采用pH梯度法制备酸性成纤维细胞生长因子(aFGF)脂质体,以包封率为考察指标,单因素筛选最优制备工艺:十八胺用量为0.02g,柠檬酸缓冲液浓度为0.03mol/L,aFGF投药量为0.04mg/mL,内外水相pH差(ΔpH)为3.5,孵育温度为35℃,孵育时间为15min。aFGF脂质体包封率为(78.82±2.56)%,Zeta电位为9.68mV,平均粒径124.03nm。透析法测定aFGF脂质体的体外释药动力学,研究结果表明aFGF脂质体与aFGF原液相比具有一定的缓释作用。稳定性研究表明,aFGF脂质体较aFGF原液稳定性得到提高,4℃贮存为佳。所制备的aFGF脂质体包封率较高,稳定性较好,具有一定的缓释作用。

Acidic fibroblast growth factors（aFGF）liposomes were prepared by pH-gradient loading.The formulation and preparation were optimized by the single-factor design regarding the encapsulation efficiency of aFGF.It consisted of stearylamine（0.02 g）,citric acid buffer（0.03 mol/L）,aFGF（0.04 mg/mL）,ΔpH（3.5）,incubation temperature（35 ℃）,and incubation time（15 min）.Under this condition,the encapsulation efficiency,Zeta potential and mean diameter of aFGF liposomes were（78.82±2.56）%,9.68 mV and 124.03 nm,respectively.Dialysis found that the release of aFGF liposomes were significantly delayed compared to aFGF solution.The stability of aFGF liposomes was greatly improved compared with that of aFGF solution,and the most appropriate storage temperature was 4 ℃.aFGF liposomes show high entrapment efficiency,good stability and significant sustained-release feature.