基于phy基因的双边界植物表达载体构建

Construction of a Plant Expression Vector Containing Two T-DNA Based on phy Gene

采用同源克隆方法从泡盛曲霉中克隆了1515bp的植酸酶基因,与黑曲霉(A.niger963)phyA2的核苷酸同源性为95%。以植物表达载体pTTBUG8为基础,构建了由35S启动子调控、具有磷高效利用功能基因(phy)的双边界植物表达载体pBSP。解决了bar基因及其产物PAT蛋白的安全性及产权等问题,并为植物养分高效利用基因工程研究奠定了重要基础。

To obtain transgenic soybean plants without selective marker genes,an efficient approach is to transform soybean with plant expression vectors containing two separate T-DNAs in a single expression vector,by which transgenic plants without selective marker genes could be selected in their progenies by crossing deletion.In this study,we used homology clone method to amplify the fragments of phytase gene from Aspergillus awamori and constructed a plant expression vector containing two T-DNAs,designated as pBSP.The results laid the basis to achieve marker-free transgenic plants in the progenies.