复硝酚钠及其组分对大豆种子萌发的影响

Effects of Sodium Nitrophenolate and its Composition on Germination of Soybean Seed

采用复硝酚钠及其有效组分邻硝基苯酚钠、对硝基苯酚钠和5-硝基愈创木酚钠对大豆浸种,通过研究发芽第1、3、5、7天的大豆种子蛋白质含量、脂肪含量、可溶性糖含量和种子发芽情况,探讨复硝酚钠及其组分对大豆种子萌发的影响。结果表明:5mg·L-1的5-硝基愈创木酚钠和10mg·L-1复硝酚钠浸种均使大豆的芽重、发芽势、发芽率、发芽指数和种子活力增加,达到了5%的显著水平。同时使种子中的储藏蛋白质从第3天分解加速,脂肪的转化速率从第5天明显加快,可溶性糖含量始终低于其它处理。以上结果表明,5-硝基愈创木酚钠在大豆种子萌发中起主要作用。

The effects of sodium nitrophenolate and its composition on germination of soybean seed were studied by soaking soybean seeds with sodium nitrophenolate,sodium ortho-nitrophenol,sodium para-nitrophenol and sodium 5-nitroguaiacolate.Seeds germinating situation,the content of protein,fat and soluble sugar of soybean seed were determined to find the key component of sodium nitrophenolate which affects the germination greatly when soybean seed germinated at 1,3,5 and 7 days,respectively.The results showed that after seeds soaked with 10 mg·L-1 sodium nitrophenolate or 5 mg·L-1 sodium 5-nitroguaiacolate the weight of sprout,germination energy,germination rate,germination index and vigor index significantly increased（P 0.05）.Furthermore,transformation of protein was accelerated from the third day;the fat in seed became decreased obviously,and the soluble sugar always was lower than other treatments from the fifth day.These results proved that sodium 5-nitroguaiacolate played an important role in the course of germination of soybean seed.