广西巴马壮族长寿老人雌激素受体β基因RsaⅠ和AluⅠ多态性研究

The research of RsaⅠ and AluⅠ polymorphisms in the estrogen receptor-β gene associated with Zhuang People centenarians of Guangxi Bama area

目的探讨雌激素受体β基因RsaⅠ和AluⅠ多态性与人类长寿的关系。方法应用PCR-RFLP技术对广西巴马156例（年龄90-105岁,平均93.2岁,长寿组）健康壮族长寿老人和142例（年龄20-72岁,平均34.8岁,对照组）健康壮族成年人的ERβ基因RsaⅠ和AluⅠ多态性进行分型,分析各种基因型频率在长寿组和对照组之间的差异及其与长寿的关系。结果 ERβ基因RsaⅠ多态性在长寿组和对照组间差异明显,长寿人群中该基因突变型明显的偏低（25.6%vs43.0%,P〈0.05）;ERβ基因AluⅠ多态性在长寿组和对照组间无差异（P〉0.05）。结论 ERβ基因RsaⅠ位点多态性与人类长寿有着较为密切的联系,其具体作用机制有待进一步研究。

Objective To explore the association between the RsaⅠ and AluⅠ Polymorphisms in the Estrogen Receptor-β gene and human longevity.Methods The Estrogen Receptor-β gene were genotyped with PCR-RFLP technique for 156 Zhuang healthily long-living people（aged 90-105,mean 93.2yrs,longevous group,LG） and 142 healthy controls（20-72,mean 34.8yrs,control group,CG） from Bama area,then the RsaⅠ and AluⅠ polymorphic genotype frequencies were analysed between the two groups.Results Analyses of the Estrogen Receptor-βgenotype showed that,any simultaneous deletion or mutation of the gene was found more frequent in CG than LG.Conclusion The RsaⅠ Polymorphisms in the Estrogen Receptor-β gene might involve in the human longevity in Bama area which mechanism needs further studies.