新型亲和膜的制备及其吸附胆红素的性能研究

Preparation of new affinity membrane and it used for adsorption of bilirubin

以尼龙膜为基膜,采用甲醛活化法偶联壳聚糖制备尼龙-壳聚糖复合膜;再用1,4-二羟基正丁烷双缩水甘油醚对其进行活化并固载聚赖氨酸作为配基,制备亲和膜。膜上壳聚糖和聚赖氨酸的含量分别为112.4和92.3mg/g尼龙膜。通过静态和动态实验测定了胆红素在膜上的吸附性能,结果表明,胆红素在膜上的吸附平衡符合Freundlich方程;在较高的温度、较低的离子强度下胆红素的去除效果较好;在动态操作条件下容易达到吸附平衡,并对结合胆红素具有较好的清除效果。

Microporous polyamide membranes were activated by formaldehyde and subsequently bound with chitosan to amplify reactive groups.Then polylysine（PLL） as ligand was immobilized onto the chitosan-coated nylon membranes.The contents of chitosan and PLL of PLL-attached membranes were 112.4 and 92.3mg/g nylon membrane,respectively.Such PLL-chitosan affinity membranes were used to adsorb bilirubin from the bilirubin-phosphate solution and albumin-bilirubin solution.The adsorption mechanism of bilirubin and the effects of temperature and ionic strength on adsorption were investigated by batch experiments.The results showed that the adsorption capacity increased with increasing the temperature and bilirubin initial concentration while decreased with increasing the NaCl concentration and the adsorption isotherm fitted the freundlich model well.The result of dynamic experiment showed PLL-attached membranes can well remove the bilirubin from the albumin-bilirubin solutions.