非水相糖苷酶高效催化的研究进展

Development of glycosidase biocatalysis in non-aqueous phase

近年来,应用糖苷酶在非水相中生物催化合成糖苷类化合物以及对糖基化合物的糖基定向改造已成为目前的一个研究热点。通过糖苷酶生物催化机理的阐述,综述了非水体系中糖苷酶催化寡糖合成与烷基糖苷合成的应用研究,尤其是耐有机溶剂糖苷酶在天然先导化合物非水相催化中的应用。非水相糖基体系为糖苷酶的高效催化及选择性催化提供了独特的环境,可望成为功能性寡糖及天然产物高效改性等领域的重要研究手段。

Application of glycosidase biocatalysis in non-aqueous phase and sugar-based directional transformation is one of the most fascinating topics in recent years.In this review,the principium of glycosidase is introduced and the application of glycoside in the synthesis of oligosaccharides and alkyl glycosides in non-aqueous system by glycosidase is summarized,particularly in catalyzing natural lead compounds with organic solvent-resistant glycosidase in non-aqueous phase,which provides a unique environment for efficient and selective catalysis,and it is desired to be an important research tool for high-performance modification of functional oligosaccharides and natural compounds in non-aqueous phase.