假升麻根乙醇提取物抑菌活性、抗氧化作用及其根中微量元素测定

Antibacterial Activity,Antioxidant Effect and Trace Element Determination of Aruncus sylvester Kostel Root

目的:对假升麻根乙醇提取物的抑菌活性、抗氧化作用进行研究,并测定假升麻根中微量元素的含量。方法:以假升麻根80%乙醇提取物为材料,采用滤纸片法对5种常见细菌的生长抑制活性、最低抑菌浓度(MIC)进行测定,并讨论温度、压力、pH值及紫外线对提取物抑菌效果的影响。采用DPPH法测定其抗氧化作用;采用原子吸收分光光度法测定其微量元素铁、锰、锌、铜的含量;采用SPSS16.0统计分析软件对实验结果进行统计分析。结果:假升麻根80%乙醇提取物对金黄色葡萄球菌的MIC为3.125mg/mL,对沙门氏菌的MIC为6.25mg/mL,对大肠杆菌、枯草芽孢杆菌和链球菌的MIC均为50mg/mL。热处理、酸性环境等对抑菌作用均无明显影响。假升麻根乙醇提取物对DPPH自由基清除的最低质量浓度为0.0025mg/mL,最高质量浓度为0.32mg/mL,其清除率达85.5%,对DPPH自由基50%抑制质量浓度为0.009mg/mL。假升麻根中微量元素Fe含量为1538.3μg/g,Mn为149.8μg/g,Zn为54.98μg/g,Cu为9.625μg/g。结论:假升麻根有着良好的抑菌效果及较强的抗氧化作用,其微量元素含量较高,具有很好的开发价值。

Objective：To investigate the antibacterial activity and antioxidant effect of Aruncus sylvester Kostel root and determine trace elements.Methods：The ethanol extract from Aruncus sylvester Kostel root was tested for its antibacterial activity against five common bacteria and minimal inhibitory concentration（MIC） by filter paper method.The effects of temperature,pressure,pH and ultraviolet light on the stability of the extract were also investigated.DPPH free radical scavenging assay was used for evaluating its antioxidant effect.Atomic absorption spectrophotometry was used to determine the contents of trace elements such as iron,manganese,zinc and copper.Data were subjected to statistical analysis using SPSS16.0 software.Results：The MICs of the extract against the tested bacteria were different.The antibacterial activity of the extract exhibited an excellent stability under various external environments.The half maximal inhibitory concentration（IC50） of the extract for scavenging DPPH free radicals was 0.009 mg/mL.The content of iron was higher than that of other detected trace elements.Conclusion：Aruncus sylvester Kostel root has excellent antibacterial activity,antioxidant effect and plentiful trace elements,which will provide promising its exploitation and utilization value.