β-葡萄糖苷酶基因工程菌发酵工艺优化

β-Glucosidase Production under Optimized Fermentation Conditions of Genetically Engineered Bacterial Strain

以菌体生物量和酶比活力为参考指标,通过单因素和响应面分析法优化β-葡萄糖苷酶基因工程菌发酵条件,得到最优的发酵条件:温度37.9℃、pH6.6、接种量10%、转速200r/min、诱导时间10h,在此条件下发酵可以使β-葡萄糖苷酶酶活力达到147.8U/L。

The fermentation conditions of genetically engineered bacterial strain for β-glucosidase production were optimized using one-factor-at-a-time method and response surface analysis based on central composite design.The optimal procedure for β-glucosidase production was based on 8 h fermentation under the conditions of pH 6.6,37.9 ℃,10% inoculation amount,200 r/min rotation speed followed by 10 h of induction with the addition of 0.5 mmol/L IPTG,which made the enzyme activity and of β-glucosidase reach up to 147.8 U/mL,respectively.