农药丙溴磷对人红细胞的毒性效应

THE NOXIOUS EFFECTS OF PROFENOFOS ON THE ERYTHROCYTE OF MAN.

目的：探讨农药丙溴磷对正常人红细胞的毒性效应。方法：采用Ｂｉａｌｓｃｈｅ试剂法检测红细胞膜唾液酸（ＲＢＣｍ－ＳＡ），化学比色法测定红细胞膜Ｃａ２＋－ＡＴＰ酶和全血中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）。结果：丙溴磷浓度在２×１０－４ｍｏｌ／Ｌ时，可明显降低ＲＢＣｍ－ＳＡ含量，并呈剂量－反应关系。丙溴磷浓度在２×１０－３ｍｏｌ／Ｌ时，红细胞膜Ｃａ２＋－ＡＴＰ酶和全血ＧＳＨ－Ｐｘ明显受抑制，其受抑制程度基本同ＲＢＣｍ－ＳＡ降低呈平行关系。结论：丙溴磷对红细胞的毒性效应与ＲＢＣｍ－ＳＡ的代谢障碍有关。

Objective:To study the noxious effects of profenofos on the erythrocyte in normal person.Methods:Bialsche reagent test was used to determine erythrocyte membrane sialic acid(RBCm SA).Chemical colorimetric method was for estimating Ca 2+ ATP ase on erythrocyte membrane and glutathione peroxidase(GSH Px) in the blood.Results:RBCm SA was decreased obviously with 2×10 -4 mol/L of profenofos concentration,and showed dose response relationship.Ca 2+ ATP ase on the erythrocyte membrane and GSH Px in the blood were inhibited with 2×10 -3 mol/L of profenofos concentration,the degree of inhibition was parallel on the whole to the decrease of RBCm SA.Conclusion:The noxious effects of profenofos on the erythrocyte are related to the metabolic disturbance of RBCm SA.