烹调油烟所致大鼠肺部病变及肺S_9中SOD、MDA变化

CHANGES OF HISTORMORPHOLOGICAL AND MDA,SOD OF LUNG TISSUES OF RATS EXPOSED TO COF.

利用动式吸入烹调油烟（浓度为（４３±４）ｍｇ／ｍ３）持续染毒６０天制备动物模型观察肺部病理形态改变，并通过分光光度法测试肺组织匀浆Ｓ９中的ＭＤＡ、ＳＯＤ，探讨其损伤机理。结果表明：ＣＯＦ染毒组肺组织病理形态改变以炎症改变为主，尚有肺泡上皮腺样化生、不典型腺样增生病变形成。阴性对照组有轻度间质性肺炎及轻度的上皮增生。Ｂ（ａ）Ｐ组肺组织炎症病变少，以鳞状上皮化生较多见。ＣＯＦ可使肺组织匀浆Ｓ９中ＭＤＡ含量增加，ＳＯＤ酶活性降低，与阴性对照组比较，差异有显著性，（Ｐ＜０．０５）。

Objective:Spranque dawley(SD) rats were exposed to subacute dose cooking oil fume(COF) in order to observe pathological changes of lung tissues.To reveal its machanisms,the concentration of malondialdehyde(MDA),the activities of superoxide dismutase(SOD) in Lung of SD rats were tests.Mathods:Rats of test group and negative group inhaled COF ((43±4)mg/m 3×30min/d×60d) and hot air (30min/d×60d) respectively,while positive control group was injected B(a)P from trachea (12mg/(kg·5d)×60d).Spectrophotometry colorimetry was made to investigate the concentration of MDA and the activties of SOD in Lung S 9 of SD rats.Results:in the study,the following feautures were noticed:The main pathological changes of test group is inflammation,alveolar epithelial hyperplasia,adenoid metaplasia and adenoid atipical hyperplasia are also found.Local mild epithelial hyperplasia and interstitiol pneumonia are found in negative control group.The main pathological canges of positive control group is squamous epithelial metaplasia in lobar and segmental bronchia.The concentration of MDA of Lung S 9 in COF and B(a)P groups are higher than in control group,whereas the activites of SOD of Lung S 9 in COF and B(a)P groups are lower than that of control group.Conclution:COF may induce adenoid metaplasia and precancerous lesions.Oxydation lesion may be one of the mechanisms of the pathological changes of lung in COF and B(a)P groups.