高效液相色谱法测定藿香正气胶囊中橙皮苷的含量

Determination Hesperidin Content of Huoxiangzhengqi Capsule by the Way of High Performance Liquid Chromatography

目的建立高效液相色谱法测定藿香正气胶囊中橙皮苷的含量。方法采用高效液相色谱方法。色谱柱为十八烷基硅烷键合硅胶为填充剂〔DiamosilTM(钻石),5.0μm,200 mm×4.6 mm〕,流动相为甲醇-0.5%醋酸溶液(30∶70),检测波长为284 nm,流速为1.0 mL/min。结果橙皮苷在5.592～74.56μg/mL范围内呈良好的线性关系,回归方程为A=21 168.38C-8 066.67,R=0.999 99。回收率为99.32%。结论该方法简单、快速、准确,能消除干扰,可用于测定藿香正气胶囊中橙皮苷的含量。

Objective To establish high performance liquid chromatography（HPLC） and assay hesperidin content of Huoxiangzhengqi capsule.Methods To use the way of HAPC.Chromato bar is octadecylsilane chemically bonded silica as bulking agent（DiamosilTM,5.0 μm,200 mm×4.6 mm）,Mobile phase is methanol-0.5% acetic acid solution（30：70）,Detected wave length is 284nm,Flow rate is 1.0 mL/min.Results Hesperidin has satisfactory linear correlation between 5.592～74.56μg/mL.Regression Equation is A=21 168.38C-8 066.67,R=0.999 99.Coefficient of recovery is 99.32%.