2007～2008年广西监测点婴幼儿人杯状病毒腹泻监测分析

Monitoring of human caliciviruse infectious in babies and infants of dairrhea in Guangxi in 2007～2008

目的了解广西某监测点2007～2008年5岁以下腹泻住院儿童杯状病毒(HuCVs)腹泻流行状况和特点。方法收集广西某监测点5岁以下急性腹泻住院儿童患者粪便标本,用RT-PCR方法检测HuCVs,部分阳性标本进行序列测定和核苷酸序列比对、进化分析病毒型别。结果 2007～2008年HuCVs平均阳性检出率为13.3%(81/607),感染高峰出现在8～10月。58株HuCVs经序列测定比对分析,全部属于GII-4型,57株为Europe-2006b株相似株。结论广西杯状病毒腹泻感染高峰较其他地区提前,GII-4型/2006b株是2007～2008年广西某监测点HuCVs腹泻的优势流行株,应对广西婴幼儿杯状病毒腹泻进行长期监测和病原分析。

Aim To monitoring the prevalence and features of human caliciviruse （HuCVs ）infectious in babies and infants of diarrhea in Guangxi （2007～2008）. Methods A total of 607 fecal samples were collected and analyzed by reverse transcription PCR. Part of caliciviruse-positive strains were selected for nucleotide analysis. Results The incidence of caliciviruse infectious was 13.3%,HuCVs infectious peak occurred from August to Noveber in 2007～2008. All of 58 sequences are GII-4 and 57 of 58 were clustered with the new GII-4 variant,termed 2006b. Conclusion The results suggests that HuCVs infectiou is common and norovirus GII-4/2006b variant is predominant in Guangxi,in 2007～2008. Long term monitoring of HuCVs is indicated .