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腺病毒载体转导绿荧光蛋白表达在海马神经元树突量化分析中的应用

Application of adenovirus-mediated green fluorescent protein expression in quantitative analysis of dendritic of hippocamcal neurons
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摘要 构建了表达绿色荧光蛋白的腺病毒(Ad-EGFP),并成功感染体外培养的海马神经元.结果表明:应用表达绿荧光蛋白腺病毒感染的海马神经元,结合荧光显微镜活细胞成像技术,能定量分析神经元树突发育过程的动态变化,可应用于神经元发育形态的动态研究及各种因素对神经元生长发育影响的研究. It was constructed the adenovirus recombinants encoding EGFP and successfully infect the cultured hippoeampal neurons by experiment. It was approved that combination of this procedure with live cell imaging and quantitative analysis could be applied to investigate the neuronal dendritic morphological development and to evaluate the effects of various factors on dendritic development.
作者 叶银萍 徐晓虹 李涛 罗清清
机构地区 浙江师范大学化学与生命科学学院 浙江师范大学生态研究所
出处 《浙江师范大学学报（自然科学版）》 CAS 2010年第3期297-302,共6页 Journal of Zhejiang Normal University:Natural Sciences
基金 国家自然科学基金资助项目(30771783 30872087) 浙江省自然科学基金重点资助项目(Z2090955)
关键词 重组腺病毒 海马神经元 树突形态 活细胞成像 recombinated adenovirus hippocampal neurons dendritic morphological live cell imaging
分类号 Q4-33 [生物学—生理学]
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参考文献22

  • 1Bertoglio L J,Joca S R,Guimares F S.Further evidence that anxiety and memory are regionally dissociated within the hippocampus[J].Behavioural Brain Research,2006,175(1):183-188.
  • 2王殿仕,李继硕.新生大鼠海马CA1神经元突触反应和树突分枝的关系(英文)[J].中国神经科学杂志,2002,18(1):400-404. 被引量：3
  • 3Chevaleyre V,Moos F C,Desarmenien M G.Correlation between electrophysiological and morphologlical characteristics during maturation of rat supraoptic neurons[J].The European Journal of Neuroscience,2001,13(6):1136-1146.
  • 4Konur S,Yuste R.Imaging the motility of dendritic protrusions and axon terminals:roles in axon sampling and synaptic competition[J].Molecular and Cellular Neurosciences,2004,27(4):427-440.
  • 5Wong W T,Wong R O.Rapid dendritic movements during synapse formation and rearrangement[J].Current Opinion in Neurobiology,2000,10(1):118-124.
  • 6Fiala J C,Feinberg M,Popov V,et al.Synaptogenesis Via Dendritic Filopodia in Developing Hippocampal Area CA1[J].Journal of Neuroscience,1998,18(21):8900-8911.
  • 7Ziv N E,Smith S J.Evidence for a Role of Dendritic Filopodia in Synaptogenesis and Spine Formation[J].Neuron,1996,17(1):91-102.
  • 8Kivell B M,McDonald F J,Miller J H.Serum-free culture of rat post-natal and fetal brainstem neurons[J].Developmental Brain Research,2000,120(2):199-210.
  • 9Chen Huanmian,Honse Y,Ikeda S R.Alternative modalities of adenovirus-mediated gene expression in hippocampal neurons cultured on microisland substrate[J].Neuroscience Letters,2004,368(2):221-225.
  • 10Breyer B,Jiang Wei,Cheng Hongwei,et al.Adenoviral vector-mediated gene transfer for human gene therapy[J].Current Gene Therapy,2001,1(2):149-162.

二级参考文献15

  • 1Sanchez C, Diaz-Nido J, Avila J. Phosphorylation of microtubuleassociated protein 2 ( MAP2 ) and its relevance for the regulation of the neuronal cytoskeleton function. Prog Neurobiol, 2000, 61 : 133-168.
  • 2Kume T, Nishikawa H, Tomioka H, et al. p75-mediated neuroprotection by NGF against glutamate cytotoxicity in cortical cultures. Brain Res, 2000, 852:279-289.
  • 3Jiang Q, Gu ZL, Zhang GY, et al. Diphosphorylation and involvement of extraceUular signal-regulated kinases (ERK1/2) in glutamate-induced apoptotic-like death in cultured rat cortical neurons. Brain Res, 2000, 857:71-77.
  • 4Tenneti L, D'Emilia DM, Troy CM, et al. Role of Caspases in N-methyl-D-aspartate induced apoptosis in cerebrocortical neurons. J Neurochem, 1998, 71:946-959.
  • 5Nicoh O, Ali C, Docagne F, et al. Neuroprotection mediated by glial ceil line-derived neurotrophic factor: involement of a reduction of NMDA-induced calcium influx by the mitogen-activated protein kinase pathway. J Neurosci, 2001,21:3024-3033.
  • 6PokornyJ,YamamotoT.PostnatalontogenesisofhippocampalCA1areainrats.I .Developmentofdendriticarborizationinpyramidalneurons[].BrainResBull.1981
  • 7BayerSA.Developmentofthehippocampalregionintherat.I .Neurogenesisexaminedwith[3H ]thymidineautoradiography[].Journal of Comparative Neurology.1980
  • 8Bayer SA,Altman J.Hippocampal development in the rat: Cytogenesis and morphogenesis examined with autoradiography and low-level X-irradiation[].Journal of Comparative Neurology.1974
  • 9Fricke R,Cowan WM.An autoradiographic study of the development of the entorhinal and commissural afferents to the dendate gyrus of the rat[].Journal of Comparative Neurology.1977
  • 10Loy R,Lynch G,Cotman CW.Development of afferent lamination in the fascia dentate of the rat[].Brain Research.1977

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浙江师范大学学报（自然科学版）
2010年 第3期

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