CoHCF 化学修饰电极与高压液相色谱联用测定脑渗析液中谷胱甘肽等巯基化合物

Determination of thiol compounds(glutathion,L-cysteine,N-acetylcysteine)in dialysis liquid of rat brain with a technique using cobalt hexacynoferrate Chemically modified electrode Coupled HPLC in vivo

本文讨论了六氰合亚铁酸钴(CoHCF)修饰电极的制备,研究了它对还原型谷胱甘肽(GSH)、L—半胱氨酸(L—Cys)及N—乙酰基半胱氨酸(NAC)的催化机理;以CoHCF修饰电极为电化学检测器与高压液相色谱联用,分离检测了GSH、L—Cys和NAC三种物质,分别在50×10^(-6)mol/L-1.0×10^(-4)mol/L、1.5×10^(-6)mol/L-2.0×10^(-4)mol/L、3.0×10^(-6)mol/L-1.0×10^(-4)mol/L浓度范围内与电流响应呈良好的线性关系,检测限分别为2.5×10^(-6)mol/L、1.0×10(-6)mol/L和1.5×10(-6)mol/L;以微渗析技术为活体取样方式,测定了大鼠脑渗析液中的谷胱甘肽等巯基化合物的含量。为电分析化学在临床医学、生理学等生命科学中的应用提供了新的途径。

A new chemically modified electrode (CME) was found to be comparable to a glass carbon electrode in terms of the eletrocatalytic of cobalt hexacyanoferrate(CoHCF) towards thiol compounds (glutathion, L-cysteine, N-acetylcysteine). Combined with high performance liquid chromatography, the CoHCF CME has good linear relationship between the concentration and peak heights of glutathion (5. 0×10-6mol/L-1.0×10-4mol/L)、L-cysteine(1.5× 10-6mol/L-2.0×10-4mol/L)、N-acetylcysteine(3.0×10-6mol/L-1.0×10-4mol/L). Coupled microdialysis sampling technique with high performance liquid chromatography-electrochemical detection (HPLC-ECD) ,we determined the glutathion content in freely moving rat brain.