骨折感染患者肠道通透性的测定及其临床意义

Detection of the intestinal permeability of infected fracture patient and its clinical significance

目的测定骨折并发感染患者肠黏膜通透性的改变,探讨骨折、骨折合并感染与肠黏膜通透性改变是否存在相关性。方法健康志愿者10名及骨折未感染、骨折感染患者各10例,予口服PEG-60010g后,收集6小时尿样;将2.00mlA液和2.00mlCHCl3混合于10ml试管中组成两相系统。将0.2mlPEG-600标准溶液或尿样加入含有二相系统的离心管中,用不含PEG的两相系统中的CHCl3相作参比,最大吸收波长处测定吸光度。结果尿样测定发现骨折并发感染组与骨折未并发感染组、对照组(健康志愿者)相比,尿液中PEG-600含量升高(P<0.05);骨折未并发感染组与对照组相比,尿液中PEG-600含量无显著性差异。结论骨折未并发感染患者肠黏膜通透性与对照组相比无显著性改变,骨折并发感染患者肠黏膜通透性增加,可能存在肠黏膜屏障功能损害。

Objective To assess the intestinal barrier function of the infected fracture patient by studying the intestinal permeability. Method The 10 infected fracture patients, the 10 fracture patients with no infection and 10 healthy volunteers were enrolled in this study. After ingestion of polyethylene glycolin 600 （PEG600）, a 6 hous-urine collection was used to analyze the amount of urinal PEG600 by spectrophotometric assay. Result The measurement of PEG600 indicated that urinal PEG600 of infected fracture patients （95.063 ±9.961 μg） were significantly higher than that of the health volunteers and non-infected fracture patients （84.970 ±9.115, 81.813± 11.102 μg, P 0.05）. Conclusion The intestinal permeability of the infected fracture patient is increased. The infected fracture patient may have intestinal barrier dysfunction.