He-Ne激光照射对SP 2/0骨髓瘤小鼠TNF及TNFR水平的影响

EFFECTS OF HE NE LASER IRRADIATION ON TNF AND TNFR LEVELS IN MICE WITH SP2/0 MARROW TUMOR

目的：观察激光照射对瘤鼠瘤组织内肿瘤坏死因子（ＴＮＦ）及肿瘤坏死因子受体（ＴＮＦＲ）的影响。方法：采用酶联免疫吸附试验及受体吸附试验分别测定瘤组织内ＴＮＦ及ＴＮＦＲ和可溶性ＴＮＦＲ（ＳＴＮＦＲ）的含量。结果：激光照射组小鼠瘤组织内ＴＮＦ活性明显增强，ＴＮＦＲ表达量增多，瘤细胞增殖率降低，与对照组相比有高度显著性差异（Ｐ＜００１）。结论：氦氖激光具有增强带瘤小鼠机体ＴＮＦ活性并促进ＴＮＦＲ表达的作用。

Objective:To observe the changes in levels of tumor necrosis factor(TNF) and TNF receptor(TNFR) in mice with tumor.Methods:The contents of TNF,TNFR and STNFR were determined in tumor tissure and tumor culture liquid by enzyme linked immunosorbent assay and ligand receptor binding assay.Results:TNF and TNFR in tumor tissure and tumor culture liquid in irradiation groups were significantly increased in amount as compared with control groups ( P <0 01) Conclusion:He Ne laser not only enhances the level of TNF,but also promotes the expression of TNFR.