摘要
随着分子生物学研究领域的发展,赖解旋酶恒温基因扩增技术(HDA)作为一种简便、快速、高效的体外恒温基因扩增技术出现。HDA依靠解旋酶解开双链DNA、结合蛋白维持单链DNA状态、DNA聚合酶催化靶片段的扩增。可以用于微生物基因组DNA、病原菌DNA、质粒DNA和cDNA等的扩增,该法具有广阔的实用前景。
With the developments in molecular biology,helicase-dependent amplification appears as a simple,rapid and efficient vitro isothermal gene amplification technology. HDA utilizes the actions of helicases to separate double-stranded DNA,the single-stranded DNA-binding protein and DNA polymerases to synthesize target fragments. It can be used for the amplifications of microbial genomic DNA,microbial DNA,plasmid DNA and complementary DNA. These features offer a great potential for practical use.
出处
《医学综述》
2010年第13期1932-1934,共3页
Medical Recapitulate
基金
福建省医学创新课题(2009-CX-5)
福建省科技创新平台建设福建省血液病学重点实验室(2009J1004)
