摘要
以抱茎獐牙菜（SwertiafranchetianaH．Smith）茎、叶为外植体，在MS＋3my／L2，4－D＋0．5mg／LBA培养基上进行培养，茎、叶出愈率分别为100％和80．4％。愈伤组织继代培养于MS＋2mg／L2，4－D＋0．5mg／LBA培养基上生长旺盛。愈伤组织转入分化培养基MS＋2mg／LBA上培养，其中48．7％形成体细胞胚。体细胞胚在无激素MS培养基上发育成苗。
Callus of Swertia franchetiana H. Smith was initiated from the stem and the leaf on MS medium supplemented with 3mg/L2. 4-D and 0. 5mg/L BA. Induction frequencies of callus were 100% and 8 0. 4 % respectively. Somaticembreyos were formed well on MS medium containing 2 mg / L BA. 48. 7% Callus produced somatic embryos. It developed into complete plants on hormonfree MS medium.
出处
《青海医学院学报》
CAS
1999年第1期10-12,共3页
Journal of Qinghai Medical College
基金
青海省教委资助
关键词
抱茎獐牙菜
组织培养
体细胞胚胎发生
再生植株
Swertia franchetiana H. Smith
Tissue culture
Somatic embryogenesis
Plant regeneration
