生物玻璃/胶原复合支架的制备及理化性能研究

Study of collagen self-assembly mechanism of regulating mineralization

以冷冻干燥和纳米合成技术制备生物玻璃和胶原复合支架材料,采用扫描电镜观察、红外光谱分析、差示扫描量热分析、热重分析、弯曲强度测试等分析手段,对复合支架的理化性能进行研究。研究结果表明:制备的支架具有多孔结构。在制备过程中,两相间产生了化学键合作用。由此论证了复合支架的孔隙结构可为细胞生长及细胞外基质的产生提供充分的空间。两相间的键合作用对于提高骨支架材料的热稳定性能、力学性能,减弱植入体内后在体液中的膨胀和浸析反应具有至关重要的作用。

A special physiological solution combined with collagen matrix was used as a mineralization system to study collagen self-assembly mechanism of regulating mineralization.The physiological solution was prepared through simulating the characteristics of body fluid.The mineralized process and mechanism of collagen matrix in vitro was observed and analyzed.The generated product in mineralized collagen was regular leaf-shaped hydroxyapatite with low crystallinity.The hydroxyapatite was characterized by strong chemical bonding with collagen macromolecules in the activation location of collagen.The self-assembly structure was regulated by interaction between collagen matrix and hydroxyapatite crystals as well as collagen＇s self-assembly ability.