甲状腺癌中VEGF表达与DNA含量、AgNOR关系的研究

CORRELATION OF THE EXPRESSION OF VEGF,DNA AND AgNOR IN THYROID CARCINOMA

目的研究血管内皮生长因子（ＶＥＧＦ）在甲状腺癌及其区域淋巴结中的表达及与癌细胞ＤＮＡ含量、ＡｇＮＯＲ的关系。方法应用免疫组织化学ＳＡＢＣ法检测１３６例甲状腺癌及淋巴结中的ＶＥＧＦ表达，同时进行细胞ＤＮＡ含量及ＡｇＮＯＲ，自动图像分析检测，１０例正常甲状腺组织作对照。结果１０例正常甲状腺组织中未发现ＶＥＧＦ的表达，１３６例甲状腺癌组织中ＶＥＧＦ阳性表达７２．９７％（９９／１３６），区域淋巴结中ＶＥＧＦ阳性表达９１％（１２４／１３６）。ＤＮＡ倍体平均值及ＡｇＮＯＲ５年以上生存组病例明显低于５年以内死亡组（Ｐ＜０．０１，Ｐ＜０．０５）。结论检测ＶＥＧＦ、ＤＮＡ含量及Ａｇ┐ＮＯＲ有助于评估甲状腺癌的生物学行为及预后。

Objective:To study the relationship of VEGF,DNA and AgNOR in human thyroid carcinoma and in regional lymph nodes.Methods:The expression of VEGF, DNA and AgNOR were studied by immunohistochemical method and image analysis system.Results:The expression of VEGF was negative in 10 cases of thyroid tissue.The positive rates of VEGF in thyroid cancer and in regional lymph node were 72.97%(99/136) and 91%(124/136)respectively.The average values of DNA and AgNOR in those survived more than 5 years groups were lower than those survived less than 5 years groups( P <0 01 and P <0 05).Conclusion:Examining the expression of VEGF,DNA and AgNOR together may benefit the estimation of patients′ prognosis.