中药蛇床子水溶性提取物中化学成分的抗诱变性研究

A STUDY ON THE ANTIMUTAGENICITY OF CHEMICAL COMPOUNDS EXTRACTED FROM CNIDIUM MONNIERI(L) CUSS USED IN CHINESE TRADITIONAL MEDICINE

本文采用Ａｍｅｓ、体内试验小鼠骨髓细胞染色体畸变和骨髓多染红细胞微核试验，对蛇床子水溶性提取物中９ 种化学成分（蛇床子素、佛手柑内酯、异虎耳草素、欧芹属素乙、花椒毒酚、花椒毒素、甲基嘧啶、尿嘧啶和１ 种待定化合物）进行了抗诱变性研究。结果表明，蛇床子素、佛手柑内酯、异虎耳草素、欧芹属素乙、花椒毒酚和花椒毒素在黄曲霉菌素Ｂ１ 诱变性的抑制作用中具有较高活性。蛇床子素、佛手柑内酯、异虎耳草素和欧芹属素乙在环磷酰胺诱发的染色体畸变和多染红细胞微核的抑制作用中也显示出较高活性。另外的３ 种成分（甲基嘧啶、尿嘧啶和１ 种待定化合物）则无抗诱变性效应。

The antimutagenicity of 9 kinds of chemical compounds (osthol, Bergaten, Isopimpinellin, Imperatorin, Xanthotoxol, Xnthotoxin, Thymine, Uracil and unknown one that need authenticating) was studied with the Ames test and the micronucleus and chromosomal aberretion assays in mice in vivo. These compounds were isolated from the water extract of Cnidium monnieri (L.) Cuss in Chinese traditional medicine. The results showed that osthol., bergapten, isopimpinellin, imperatorin, xanthotoxol and xanthotoxin had inhibited activity of mutagenicity of aflatoxin B\-1. And osthol, bergapten, isopimpinellin, imperatorin also had high activity in inhibiting the chromosomal aberrations and micronuclei that induced by cyclophosphamid in vivo. The other three compounds (Thymine, Uracil and unknown one that need authenticating) showed no antimutagenicity.