海带多糖对环磷酰胺诱发小鼠骨髓细胞微核率的抑制作用

INHIBITORY EFFECT OF LAMINARIA JAPONICA POLYSACCHARIDES ON MICRONUCLEUS RATE INDUCED BY CYCLOPHOSPHAMIDE IN MICE

本文以小鼠骨髓嗜多染红细胞微核率（ ＭＮＦ） 为指标初步探讨了海带多糖（ＬＰＳ） 对环磷酰胺（ ＣＰ） 诱发的小鼠染色体突变的拮抗作用。结果显示：阳性组环磷酰胺（ ＣＰ４０ ｍｇ／ｋｇ．ｂｗ） 与海带多糖组（ ＬＰＳ５００ ｍｇ／ｋｇ．ｂｗ 、１０００ ｍｇ／ｋｇ ．ｂｗ 、２０００ ｍｇ／ｋｇ ．ｂｗ） 微核率分别为２８‰、２２ ‰、１２ ‰、１１ ‰，海带多糖组与阳性组相比较差异显著（ Ｐ＜０ ．０１） ，由此可见，海带多糖能明显抑制环磷酰胺诱发的小鼠体细胞的遗传损伤。

Inhibitory effect of Laminaria japonica polysacchrides (LPS) on micronucleus rate induced by cyclophosphamide (CP) had been studied by mean of micronucleus test in mouse bone marrow polychromatic erythrocyte (PCE). Micronucleus rates of positive control group (CP 40mg/kg.bw), and three LPS tested groups (LPS 500mg/kg, 1000mg/kg, 2000mg/kg) was 28‰, 22‰, 12‰, 11‰, respectively. There were significant differences tested groups (1000mg/kg, 2000mg/kg) as compared with positive control group. The results showed that LPS possessed inhibitory effects on mutagenesis indued by cyclophamide.