头孢氨噻肟在妊娠大鼠的胎盘转运

Placental Transfer and Pharmacokinetics of Cefotaxime in Pergnant Rats

目的：建立一个大鼠的胎盘转运模型，研究头孢氨噻肟（CTX）在大鼠的胎盘转运及药动学。方法：用孕20天SD大鼠，分别从母体面和胎鼠面给药。母体给药经母鼠颈静脉注射CTX（100mg／kg），胎鼠给药依次经胎鼠腹腔注射CTX5mg／0．05ml，分别取母血、胎血及羊水，用HPLC法测定药物浓度。将母─胎单位设想为一个简单的二室开放模型（一个胎儿室，一个母亲室），分别计算母胎鼠的药动学参数及药物的母体胎盘清除率（CLmf）和胎鼠胎盘的药动学参数（CLfm）。结果：母鼠和脂鼠给药后消除半衰期和分布容积分别为：33min、37min和0．54L／kg、0．18L／kg，CLmf和CLfm分别为0．043L／kg·min和0．088L／kg·min。结论：本实验结果与以往结果相似，说明本模型基本可靠，为今后研究胎盘转运提供了一个可利用的方法。

Objectives: set up an animal medel for studying placental transfer and phrmacokinetics of CTX.Methods: Twenty days pregnant SD strain ratS were randomized, and received a single dose of CTX by one of the following routes: 1. matemal iv 100mg/kg weight. 2. fetal ip. 5mg/0. 05ml. We supposed matemal - fetalunit is a simple two - compartment model(one matemal Pool and one fetal pool). After CTX was administered, we determained CTX comcernttation of matemal and fetal blood plasm and amniotic fluid by HPLC method:We also determined the blood plasm in matemal and fetal.Results: The placental clearance from matemal to fetus(CLmf) and from fetus to matermal (CLfm) were0. 043L/kg/minand 0. 088L/kg/min respectively. The other pharmacokinetic parameters were also calculated: in matemal, elimination half- life was 33 min, distribution volume was 0. 54L/kg, in fetus they were 37 minute and 0. 18L/kg. The ratio of fetal - to matemal plasm CTX concemtaion was 0. 33 following a bolus administration to fetus and mother.Conclusion: We set up a reliable animal in situ model, and supply a method for calculating placental transfer.