利心冲剂对心力衰竭大鼠模型的氧化应激和凋亡相关基因的影响

Effect of Lixin Granule on the Related Gene of Apoptosis and Oxidiative Stress of Adriamycin-induced Heart Failure in Rats

目的探讨利心冲剂调控心力衰竭大鼠模型氧化应激抗细胞凋亡的分子机制。方法实验分组:正常组、模型组、卡托普利治疗组、利心冲剂低剂量组、利心冲剂高剂量组。腹腔注射阿霉素(ADR)5mg/kg,每5天1次,共3次,复制大鼠心力衰竭模型,左心室插管术测定血流动力学指标;血清学检测氧化指标SOD1、MDA;RealtimePCR检测氧化应激及凋亡相关基因SOD1、PGC-1α和Bcl-2、Bax。结果利心冲剂治疗组和模型组比较,形态学显示心肌细胞变性坏死明显减轻;左心室收缩内压显著升高;氧化应激相关指标SOD1、PGC-1α升高、MDA降低;凋亡相关基因Bcl-2升高、Bax/Bcl-2降低;均有显著性差异(P<0.05)。结论利心冲剂调控氧化应激相关基因SOD1、PGC-1α,降低凋亡相关基因Bax/Bcl-2表达,减轻心肌细胞凋亡,改善心脏收缩与舒张功能。

ObjectiveTo observe the protective effect of Li xin granule on the related gene of apoptosis of rats with adriamycin-induced heart failure. MethodsSixty adult male Wistar rats were divided into 5 groups： normal control group, adriamycin（ADR） group,low-Li xin granule group, high-Li xin granule group, captopril group. Then ADR of 5mg/kg was given intraperitoneally to copy the model of heart failure,once for 5 days.The biological signal collecting system was used to record and analyze the LVSP of the rats. The pathological changes of the cardiomyocytes were observed. Real time PCR was used to record and analyze the related gene of apoptosis （Bcl-2mRNA）and oxidiative stress（SOD1,PGC-1α） of rats. ResultsAs compared with NC group, the LVSP of the ADR group was significantly lower （P〈0.05）, but LVSP of the high-Li xin granule group was markedly higher than that of the ADR group （P〈001）.The results of HE staining showed that the myocardial tissue in CHF group was injury.ConclusionH- Li xin granule treatment can relieve apoptosis of ADR on myocardium and also obviously improve the cardiac contractility of heart failure rats.