肌球蛋白Ⅵ定向运动的蒙特卡罗模拟

Monte carlo simulation of the directional motion of myosin Ⅵ

根据myosin Ⅵ分子马达头部核苷的变化建立一个四态循环,然后利用蒙特卡罗方法模拟分子马达的动力学性质,讨论了myosin Ⅵ速度、滞留时间与溶液中ATP浓度、ADP浓度和负载力F的关系。模拟得到:myosin Ⅵ会在轨道微丝上进行梯跳运动并且每次在微丝上的滞留时间并不相同;另外,myosin Ⅵ的速度会随着ATP浓度的增加而增大,随着ADP浓度和负载力的增加而减小;负载力对myosin Ⅵ在胞内执行输运还是连接功能起一定的调节作用。

According to the transformation of nucleotide in the motor domain of myosin Ⅵ,a circular model of four states as former some models was found.By using Monte Carlo method to simulate the dynamic characters basing on chemical reaction rates between two states,the relations among the displacement,the velocity,the dwell time of myosin Ⅵ with concentration of ATP,ADP and backward load F were analyzed.The dwell time of myosin Ⅵ during every cycle is different.The velocity of myosin Ⅵ would be faster when concentration of ATP increased,and slower when concentration of ADP increased.Otherwise backward load F might play an adjustive function between translocation and anchoring in cell.