用基因融合技术进行杀蚊幼毒蛋白基因的研究 Ⅰ.球形芽孢杆菌毒蛋白基因42的克隆

STUDIES ON THE LARVICIDAL TOXIC PROTEIN GENE WITH THE TECHNIQUE OF GENE FUSION Ⅰ. CLONING OF GENE 42 OF BACILLUS SPHAERICUS

为了提高球形芽孢杆菌对中华按蚊的毒效，采用聚合酶链反应（ＰＣＲ）技术成功扩增了Ｂ．ｓ．中有毒效作用的基因片段，通过Ｔ４ＤＮＡ聚合酶的修饰，使目的基因片段得以成功克隆。借助光敏生物素核酸标记探针，原位杂交筛选出目的基因阳性克隆。

Aiming at improving the toxicity of Bacillus sphaericus (B.s.) against Anopheles sinensis , we succeeded in amplifying the toxic gene fragment of B.s. with the help of PCR and getting its clone with the decoration of T 4DNA polymerase. The positive clones were screened by in situ hybridization with the probe which was labelled with biotin. In this study, T 4DNA polymerase played an important role in cloning the PCR products.