复合酶法制备乌桕梓油生物柴油工艺优化

Optimization of biodiesel production by combined lipases-catalyzed transesterification from stillingia oil in an organic solvent medium

采用响应面法对复合酶Novozym435和Lipozyme TLIM催化乌桕梓油制备生物柴油的工艺条件进行了优化。通过SAS 9.0软件分析,获得最优工艺参数为:复合酶加入量Novozym 4352.52%、Lipozyme TLIM1.68%(基于油质量),反应温度40℃,叔丁醇加入量41%(基于油体积),分子筛加入量5.5%(基于油质量),醇油摩尔比5.05∶1。在此条件下,生物柴油的转化率高达98.72%,与回归模型预测的最优条件下生物柴油转化率97.69%非常接近。

The biodiesel production technology catalyzed by combined lipases of Novozym 435 and Lipozyme TLIM from stillingia oil was optimized using response surface methodology（RSM）.The optimal conditions were as follows： Novozym 435 dosage 2.52%,Lipozyme TLIM dosage 1.68%（based on oil weight）,reaction temperature 40℃,molar ratio of methanol to oil 5.05∶1,tert-butanol dosage 41%（based on oil volume）,molecular sieves 5.5%（based on oil weight）.Under the optimal conditions,the biodiesel yield achieved 98.72%,which was closed to the predicted value 97.69%.