化学合成的几丁寡糖及其结构类似物诱导烟草对黑胫病抗性研究

Induced resistance of tobacco to black shank disease by synthesized β-1,3-N-acetyl-glucosamine oligosaccharides and β-1,4-N-acetyl-glucosamine oligosaccharides

为了探讨寡糖诱导物的分子结构与诱导抗病性之间的相互关系,以7个化学合成的寡糖为诱导物,研究了烟草植株对黑胫病的诱导抗性。结果表明,-β1,3-乙酰氨基葡聚四糖、-β1,4-乙酰氨基葡聚三糖和-β1,4-乙酰氨基葡聚四糖的诱导处理对烟草黑胫病表现活体抗性,相对诱导效果分别为62.5%,50.0%和75.0%;-β1,3-乙酰氨基葡聚四糖、-β1,4-乙酰氨基葡聚三糖和-β1,4-乙酰氨基葡聚四糖的诱导处理对烟草黑胫病表现离体抗性,相对诱导效果分别为56.25%,50.0%和62.5%。研究结果表明寡糖的聚合度以及寡糖主链的糖苷键连接方式可能是影响诱导抗病性的重要因子,不同浓度寡糖处理后烟草的诱导抗病性有差异。

To explore the relationship between oligosaccharides molecular structure and induced disease resis-tance of plants,induced resistance of tobacco to black shank disease with seven synthesized oligosaccharides as elicitors was studied.The tobacco plants induced by β-1,3-N-acetyl-glucosamine tetrasaccharide,β-1,4-N-acetyl-glucosamine trisaccharide and β-1,4-N-acetyl-glucosamine tetrasaccharide showed resistance to the disease with relative efficiency of 62.5%,50.0% and 75.0%,respectively.The tobacco detached leaves induced by β-1,3-N-acetyl-glucosamine tetrasaccharide,β-1,4-N-acetyl-glucosamine trisaccharide and β-1,4-N-acetyl-glucosamine tetrasaccharide showed resistance to the disease with relative efficiency of 56.25%,50.0% and 62.5%,respectively.It was suggested that the degree of polymerization and chain backbone linkage structures of oligosaccharides might be the important factors affecting the induced disease resistance.Moreover,the induced disease resistance by oligosaccharides varied with treated oligosaccharide concentrations.