摘要
Using degenerate PCR and TAIL-PCR,a protein kinase gene OPK1(Genebank accession No.:EU417815) was cloned from mycoparasite fungi Olpitrichum tenellum.OPK1 has an open reading frame of 2 031 bp interrupted by two introns(108 bp,84 bp) and putatively encodes a protein of 676 aa.Phylogenetic analysis indicated that OPK1 was most similar to other serine-threonine protein kinase in fungi.Southern blot analysis indicated that OPK1 is present as a single copy in the genome.RT-PCR showed it could be transcribed both in the phase of spore germination and hyphal growth.
Using degenerate PCR and TAIL-PCR,a protein kinase gene OPK1（Genebank accession No.：EU417815） was cloned from mycoparasite fungi Olpitrichum tenellum.OPK1 has an open reading frame of 2 031 bp interrupted by two introns（108 bp,84 bp） and putatively encodes a protein of 676 aa.Phylogenetic analysis indicated that OPK1 was most similar to other serine-threonine protein kinase in fungi.Southern blot analysis indicated that OPK1 is present as a single copy in the genome.RT-PCR showed it could be transcribed both in the phase of spore germination and hyphal growth.
出处
《植物病理学报》
CAS
CSCD
北大核心
2010年第3期329-332,共4页
Acta Phytopathologica Sinica
基金
国家自然科学基金(30571246)
山东省教育厅项目(J08LF56)
