摘要
The gene of RNA-dependent RNA polymerase(RdRP) was cloned by RT-PCR from Sugarcane yellow leaf virus-Fuzhou isolate(CHN-FJ1) and then cloned into pMD18-T vector.The sequence showed that the fragment comprised 1 212 nucleotides including part gene of ORF1 and ORF2.The ORF2 was involved the RdRP gene consisted of 995 nucleotides and encoded putative protein of 331 amino acids.Compared the nucleo-tide sequence and encoded putative protein of CHN-FJ1 with the other isolates from different countries,they shared the homology above 92.0%.Phylogenetic tree suggested that the sixteen isolates were classified into four types according to the amino acid sequence of the RdRP.One of the groups contained CHN-FJ1 and the other isolates from China,American,Brazil,Australia and Colombia;however,there was the closest relation between CHN-FJ1 and BRA-YL1 isolate from Brazil.
The gene of RNA-dependent RNA polymerase（RdRP） was cloned by RT-PCR from Sugarcane yellow leaf virus-Fuzhou isolate（CHN-FJ1） and then cloned into pMD18-T vector.The sequence showed that the fragment comprised 1 212 nucleotides including part gene of ORF1 and ORF2.The ORF2 was involved the RdRP gene consisted of 995 nucleotides and encoded putative protein of 331 amino acids.Compared the nucleo-tide sequence and encoded putative protein of CHN-FJ1 with the other isolates from different countries,they shared the homology above 92.0%.Phylogenetic tree suggested that the sixteen isolates were classified into four types according to the amino acid sequence of the RdRP.One of the groups contained CHN-FJ1 and the other isolates from China,American,Brazil,Australia and Colombia;however,there was the closest relation between CHN-FJ1 and BRA-YL1 isolate from Brazil.
出处
《植物病理学报》
CAS
CSCD
北大核心
2010年第3期333-336,共4页
Acta Phytopathologica Sinica
基金
农业部"948"计划项目(2006-G37)
公益性行业科研专项(3-9)
现代农业产业技术体系建设专项资金
