虎源猫瘟热病毒VP2蛋白特异性单克隆抗体的制备

Development and Identification of the Monoclonal Antibodies Against Recombinant VP2 Protein of Feline Panleukopenia Virus from Tiger

以原核表达的虎源猫瘟热病毒(FPV)VP2蛋白作为免疫抗原,免疫6周龄BALB/c小鼠,用FPV全病毒作为筛选抗原,采用淋巴细胞杂交瘤技术,经过有限稀释法获得了1株可稳定分泌抗虎源FPVVP2蛋白的杂交瘤细胞株3C4。间接ELISA方法测定3C4株单抗腹水效价为1∶12800,亚类鉴定为IgG2a类型,间接免疫荧光试验显示该株单抗能与虎源FPV发生反应,而免疫印迹试验该株单抗未见特异性反应带。

Monoclonal antibody（McAb）3C4 against recombinant VP2 protein of feline panleukopenia virus from tiger were developed by fusing myeloma cell line SP2/0 with splenocytes of BALB/c mice immunized with prokaryotic expressed VP2 protein.The positive hybridoma cells were sifted by ELISA with tiger feline panleukopenia virus.The antibody titers of ascites for the hybridoma lines were 1∶12 800.The subtype of monoclonal antibodies was determined as IgG2a.Indirect immunofluorescent test indicated that the monoclonal antibody could specifically react with FPV,and it may be used as an valuable tools for diagnosis of FPV.