双标记法检测乙型和丙型肝炎病毒双重感染的肝组织内病毒标志

Detection of viral markers in liver tissue dually infected with hepatitis B and C viruses by double labelling

目的研究乙型肝炎病毒（ＨＢＶ）与丙型肝炎病毒（ＨＣＶ）双重感染者肝组织内两种病毒的分布特点和相互关系。方法用免疫组化和原位杂交法分别检测了ＨＢＶ的核酸及抗原和ＨＣＶ的核酸及抗原，对其中乙型肝炎核心抗原，对其中乙型肝炎核心抗原（ＨＢｃＡｇ）与ＨＣＶＲＮＡ同时阳性的组织进一步做了同一切片的双标记染色。结果ＨＢＶ和ＨＣＶ感染细胞大多各自分布于不同的肝小叶或假小叶内；即使共存于同一小叶时，多数并不感染同一细胞。结论在乙型和丙型肝炎病毒双重感染组织中，两种病毒在多数细胞内并不直接发生相互干扰；双标记法有助于分析重叠感染组织中病毒之间的相互关系。

Objective To study the distribution and mutual relation of HBV and HCV in dually infected liver tissue.Methods Detected HBV and HCV nucleic acids and their antigens by immunohistochemistry and in situ hybridization, the cases with HBcAg and HCV RNA positive were tested further on single section by double labeling. Results On the section tested by double labeling we could see that positive hepatocytes infected with HBV or HCV were mostly present in different liver lobules, even if the two virues were seen in the same lobule, most of them were not seen within a single cell.Conclusions In most of hepatocytes, there was no direct interference or suppression of each other in the liver tissue dually infected with HBV and HCV. Double labeling method is uesful to analyze the coinfection of two viruses in the same tissue.