摘要
目的研究沈阳地区丙型肝炎病毒(HCV)基因型分布状况及其临床意义。方法应用聚合酶链反应(PCR)方法对沈阳地区84例血清抗-HCV及HCVRNA均阳性的各临床型丙型肝炎患者进行HCV基因分型。结果84例丙型肝炎中,HCV-Ⅱ型45例(53.6%),HCV-Ⅲ型26例(30.9%),Ⅱ/Ⅲ混合型13例(15.5%);HCV不同基因型在各型丙型肝炎中总体分布不一致,经统计学处理,P<0.05;在急性肝炎、慢性肝炎轻度、中度、重度及肝炎后肝硬化中,HCV-Ⅱ型感染分别占55.6%,36.2%,75.0%,85.7%及88.9%;HCV-Ⅲ型感染分别占22.2%,46.8%,8.3%,0%骸11.1%。结论沈阳地区HCV感染以Ⅱ型为主,其次为Ⅲ型及Ⅱ/Ⅲ混合型;HCV基因型与丙型肝炎严重程度密切相关。
Objective To study the distribution of HCV genotypes in different clinical types of hepatitis C in Shenyang area and its clinical significance.Methods The HCV genotypes of 84 HC patients of different clinical types were detected by PCR assay with four kinds of type specific primers.Results In 84 patients with hepatitis C, the HCV Ⅱ and HCV Ⅲ genotypes and the Ⅱ/Ⅲ mixed genotype were 53.6%,30.9% and 15.5% respectively. The distribution of HCV genotypes in different HC clinical types was not identical, P <0.05. In acute hepatitis, in mild, moderate and severe degree of chronic hepatitis, and in liver cirrhosis, the infection rates of genotype Ⅱ HCV were 55.6%,36.2%,75.0%,85.7% and 88.9% respectively, but that of genotype Ⅲ were 22.2%,46.8%,8.3%,0% and 11.1% respectively.Conclusion Infection of geno type Ⅱ HCV was predominant in Shenyang area followed by HCV Ⅲ and Ⅱ/Ⅲ mixed geuotypes. The different genotypes of HCV were related to the severity of hepatitis C.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1999年第1期64-67,共4页
Chinese Journal of Experimental and Clinical Virology
基金
辽宁省科研基金