沈阳地区各型丙型肝炎患者丙型肝炎病毒的基因分型

Study on HCV genotypes in different clinical types of hepatitis C patients in Shenyang area

目的研究沈阳地区丙型肝炎病毒（ＨＣＶ）基因型分布状况及其临床意义。方法应用聚合酶链反应（ＰＣＲ）方法对沈阳地区８４例血清抗－ＨＣＶ及ＨＣＶＲＮＡ均阳性的各临床型丙型肝炎患者进行ＨＣＶ基因分型。结果８４例丙型肝炎中，ＨＣＶ－Ⅱ型４５例（５３．６％），ＨＣＶ－Ⅲ型２６例（３０．９％），Ⅱ／Ⅲ混合型１３例（１５．５％）；ＨＣＶ不同基因型在各型丙型肝炎中总体分布不一致，经统计学处理，Ｐ＜０．０５；在急性肝炎、慢性肝炎轻度、中度、重度及肝炎后肝硬化中，ＨＣＶ－Ⅱ型感染分别占５５．６％，３６．２％，７５．０％，８５．７％及８８．９％；ＨＣＶ－Ⅲ型感染分别占２２．２％，４６．８％，８．３％，０％骸１１．１％。结论沈阳地区ＨＣＶ感染以Ⅱ型为主，其次为Ⅲ型及Ⅱ／Ⅲ混合型；ＨＣＶ基因型与丙型肝炎严重程度密切相关。

Objective To study the distribution of HCV genotypes in different clinical types of hepatitis C in Shenyang area and its clinical significance.Methods The HCV genotypes of 84 HC patients of different clinical types were detected by PCR assay with four kinds of type specific primers.Results In 84 patients with hepatitis C, the HCV Ⅱ and HCV Ⅲ genotypes and the Ⅱ/Ⅲ mixed genotype were 53.6%,30.9% and 15.5% respectively. The distribution of HCV genotypes in different HC clinical types was not identical, P <0.05. In acute hepatitis, in mild, moderate and severe degree of chronic hepatitis, and in liver cirrhosis, the infection rates of genotype Ⅱ HCV were 55.6%,36.2%,75.0%,85.7% and 88.9% respectively, but that of genotype Ⅲ were 22.2%,46.8%,8.3%,0% and 11.1% respectively.Conclusion Infection of geno type Ⅱ HCV was predominant in Shenyang area followed by HCV Ⅲ and Ⅱ/Ⅲ mixed geuotypes. The different genotypes of HCV were related to the severity of hepatitis C.