肾综合征出血热患者软腭粘膜对乳鼠致病性的研究

The pathogenicity of HFRS patient′s soft palate mucosa to suckling mice

目的探讨肾综合征出血热（ＨＦＲＳ）的发病机理。方法２～３天的近交系Ｂａｌｂ／Ｃ乳鼠，脑内接种早期ＨＦＲＳ重症患者软腭粘膜出血点组织悬液，用套式聚合酶链式反应检测乳鼠脑、肺及肾组织中的ＨＦＲＳ病毒ＲＮＡ。结果接种乳鼠发病死亡，剖检显示，发病乳鼠各器官呈现明显微血管系统和实质组织病变，乳鼠脑、肺及肾组织中ＨＦＲＳ病毒ＲＮＡ阳性。结论早期ＨＦＲＳ患者软腭粘膜出血点组织中含有ＨＦＲＳ病毒。

Objective To expiore the pathogenic mechanism of hemorrhagic fever with renal syndrome(HFRS).Methods 2 3 days old inbred Balb/C suckling mice were inoculated intracerebrally with tissue suspension of soft parate petechia of HFRS patients and the HFRS virus RNA was tested in brain, lung and kidney tissues of sucking mice by nested PCR.Results Some of the mice developed acute disease and died. The autopsy analysis indicated that these mice showed pathological changes in system microvascular and parenchymatous tissues. HFRS virus RNA was detected in their brains, lungs and kidney tissues.Conclusions These results indicate that HFRS virus exists in the tissue of petechial hemorrlngic spots on soft palate petechia of HFRS patients.