肝细胞癌组织Smac蛋白表达及其与细胞凋亡和增殖的关系

Expression of Smac protein and its relationship with cell apoptosis and proliferation in hepatocelluar carcinoma tissues

目的:探讨肝细胞癌(HCC)组织中Smac(第二个线粒体Caspase激活因子)的表达及其与细胞凋亡和增殖的关系。方法:应用免疫组化二步法检测HCC及相应癌旁肝硬化组织各41例、肝血管瘤旁正常对照组织9例中Smac、增殖细胞核抗原(PCNA)的表达;应用TUNEL法检测组织中细胞的凋亡。结果:在41例HCC组织中,Smac阳性数为14例(34.14%),相应癌旁肝硬化组织为23例(56.10%),肝血管瘤旁正常对照组织为7例(77.78%),HCC组织中Smac的阳性率与相应癌旁及正常组织相比差异有统计学意义,χ2值分别为3.989和4.115,P值分别为0.046和0.042;HCC组织中Smac蛋白的表达与细胞的凋亡增殖比有相关性,t′=2.26,P<0.05,而与患者的年龄、组织分化程度等临床病理指标均无相关性,P均>0.05。结论:Smac的相对低表达或表达缺失在一定程度上打破了HCC组织中细胞凋亡与增殖之间的动态平衡,在HCC的形成过程中起到了重要的作用。

OBJECTIVE：To investigate the expression of Smac protein in human hepatocelluar carcinoma and their relationship with cell apoptosis and proliferation.METHODS：The expression of Smac and the proliferating cell nuclear antign （PCNA） in 41 cancer tissues,41 adjacent cirrhosis tissues and 9 normal control tissues in hemangioma were assessed by the two-step immunohistochemical method and the apoptosis was detected by the TUNEL method.RESULTS：Smac proteins were expressed in 14（34.14%） of the 41 cases of hepatocelluar carcinoma,in 23（56.10%） of the 41 cases of the adjacent cirrhosis tissues,and in 7（77.78%） of the normal tissues in hemangioma.The smac protein positive expression rate in hepatocelluar carcinoma was significantly higher than that in the adjacent cirrhosis tissues and the normal control tissues,χ^2 values were 3.989 and 4.115,P values were 0.046 and 0.042.The Smac protein expression in cancer was significantly correlated with the ratio of apoptic index to proliferative index （t′=2.26,P〈0.05）,but was not with the clinicopathological indicators such as the age and histological grade （P〈0.05）.CONCLUSION：The relatively lower level of the expression of Smac may in a certain extent break the dynamic balance between apoptosis and proliferation of hepatocelluar carcinoma cells,then play an important role in the pathogenesis of hepatocelluar carcinoma.